---
title: DNA Template Calculator
---

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## DNA Template Calculator

Calculate the amount of DNA template required for your NTxscribe® *in vitro* transcription (IVT) reaction.

Enter your linearized DNA template length and concentration. The calculator will determine the amount of DNA template required per NTxscribe® run.

Linearized DNA Template Length (bp)

DNA Template Concentration (mg/mL)

i All other parameters are set to default values for NTxscribe® reactions.

DNA Template Required  
in milligrams per NTxscribe® Run

— mg

DNA Template Required  
in mL per NTxscribe® Run

— mL

### NTxscribe® Reaction Mix *(per run)*

| Reagent | Amount (mL) | Final Concentration |
| --- | --- | --- |
| Nuclease-free water | — | N/A |
| NTxscribe® Buffer | 1.7 | 1x |
| Cap Analog (optional) | 0.68 | 4 mM |
| DNA Template | — | 0.05 µM |
| NTxscribe® Enzyme Mix 1 | 3.4 | 2x |
| Total Reaction Volume | 17 |   |

#### Creating the fast lane for vital therapeutics.

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NTx is enabling the future of medicine with cell-free biomanufacturing systems that drive breakthroughs in research, therapeutics, and personalized cancer vaccines. The company’s flagship NTxscribe platform brings industrial-grade RNA manufacturing to the benchtop, producing high-purity, high-integrity RNA with unmatched speed, efficiency, and scalability – all within a fully closed system. NTxscribe’s single-use RNA cartridges run on NTxbiomaterials, a proprietary line of reagents that are manufactured in the U.S. with fully domestic raw materials.

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