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High-Integrity mRNA & saRNA
in Hours, Not Days
Meet NTxscribe®, the automated, continuous flow platform transforming RNA synthesis
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Go from DNA template to 50mg* purified, high-quality mRNA or saRNA in just 3 hours. Accelerate your research and development with unprecedented speed, scalability, and benchtop accessibility.
*Output varies based on template size, typical range is 40-65mg
Automated RNA Synthesis & Purification
To support the growing demand for mRNA based vaccines and therapeutics, NTx has developed a first of its kind, proprietary continuous-flow, in-vitro, transcription system utilizing hollow fiber bioreactors. This biosynthetic process provides a low cost and rapidly deployable vertically integrated manufacturing system. This system allows for manufacturing scale from milligram R&D quantities to large scale commercial needs all in a small, economical footprint, with the supply of critical enzymatic components secured by NTx.
In our standard workflow, 5’-capping of the mRNA is accomplished co-transcriptionally in continuous flow (directly coupled mRNA synthesis).
This unique approach minimizes handling of the material in between production steps, prevents damaging the molecules and ensures integrity, incorporates a purification process that improves yields, and can be scaled up linearly using the same process from discovery through product commercialization, saving valuable time.
NTxscribe® Process (IVT + Purification)
The Principal Advantages of NTxscribe® Include:
- Higher RNA integrity out of IVT compared to batch reactions due to short residence time in bioreactor - reducing burden on downstream purification
- Complete set of NTx enzymes for mRNA production
- Single-use, closed system configuration to ensure product sterility
- Decreased shear and handling, minimizing product degradation and contamination
- Streamlines manufacturing operations by reducing batch unit operations
- Enables linear scaling from mg to gram scale
- Benchtop modular design can accommodate different drug substance formats in small scalable footprint
- Allows for integration of in-line process analytics
Producing Conventional and Self-Amplifying mRNA
NTxscribe® consistently delivers high-quality RNA across a broad range of transcript sizes, from standard mRNA to challenging self amplifying RNA (saRNA). Our continuous flow technology excels where batch methods often struggle, preserving the integrity of long and sensitive molecules. Whether you’re optimizing vaccine candidates or developing novel therapeutics, trust NTxscribe® to reliably produce the high-integrity RNA essential for your success.