Fully Recombinant In Vitro Protein Manufacturing
First and only fully recombinant cell-free expression system to produce proteins and minimize unwanted cellular metabolism
Extract-based in vitro systems are challenged by:
- Residual metabolic activity lowering efficiency (nucleotide and amino acid recycling)
- Presence of cellular nucleases that degrade input DNA
- Proteases that degrade target protein
- Poor reproducibility
NTx has developed a fully recombinant, thermostable, reliable, and functional in vitro transcription/translation system for the production of biologics.
Our Recombinant In Vitro Technology
NTx Cell-Free Expression:
- Minimizes unwanted cellular metabolism
- Supports linear or circular DNA templates
- Efficient ATP regenerating system to supply sustained energy
- High reproducibility
- Generate target proteins in less than a day
vs.
Extract-Based Cell-Free Expression:
- Cell extracts from E. coli, wheat germ, rabbit reticulocyte, insect cells
- Mostly circular DNA templates
- Energy regeneration based upon ATP present in lysate
- Limited run times
- Poor reproducibility
Our Process
In comparison to cell-based expression, cell-free (CF) production of biologics offers enhanced flexibility, quicker response times, reduced energy consumption, and a smaller facility footprint. CF production does not require dedicated facilities per strain/product and can be scaled throughout the commercialization process saving time and money.
NTxpress® is designed to offer high-quality linear scaling with compressed cycle time for product manufacturing. The NTxpress® platform requires limited upfront capital investment and can be adapted for a turnkey modular solution.