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NTXpress

Pure, Scalable Protein Synthesis.
On Demand.

Fully Recombinant In Vitro Protein Manufacturing

Introducing NTxpress®—the future platform for cell-free protein synthesis. Leveraging a fully recombinant system, NTxpress® is designed to deliver high-yield, high-purity proteins with speed and scalability, free from the limitations of traditional cell-based or extract-based methods. NTx has developed a fully recombinant, thermostable, reliable, and functional in vitro transcription/translation system for the production of biologics.

First and only fully recombinant cell-free expression system to produce proteins and minimize unwanted cellular metabolism

Extract-based in vitro systems are challenged by:

  • Residual metabolic activity lowering efficiency (nucleotide and amino acid recycling)
  • Presence of cellular nucleases that degrade input DNA
  • Proteases that degrade target protein
  • Poor reproducibility

The NTxpress® Advantage: Purely Recombinant Technology

Extract-Based Cell-Free Expression

  • Cell extracts from E. coli, wheat germ, rabbit reticulocyte, insect cells
  • Mostly circular DNA templates
  • Energy regeneration based upon ATP present in lysate
  • Limited run times
  • Poor reproducibility

NTx Cell-Free Expression

  • Minimize unwanted cellular metabolism
  • Supports linear or circular DNA templates
  • Efficient ATP regenerating system to supply sustained energy
  • High reproducibility
  • Generate target proteins in less than a day

Streamlined, Scalable Protein Production

In comparison to cell-based expression, cell-free (CF) production of biologics offers enhanced flexibility, quicker response times, reduced energy consumption, and a smaller facility footprint. CF production does not require dedicated facilities per strain/product and can be scaled throughout the commercialization process saving time and money.

NTxpress® is designed to offer high-quality linear scaling with compressed cycle time for product manufacturing. The NTxpress® platform requires limited upfront capital investment and can be adapted for a turnkey modular solution.