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Vertically Integrated Continuous Flow mRNA Manufacturing Solution

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Discover NTxscribe®: our benchtop, plug and play continuous flow mRNA IVT and purification system that produces 10-50 mg of high integrity mRNA in 3 hours
 
Secure your system with a $25,000 deposit. The deposit amount will be credited towards the purchase of the NTxscribe system.
 
Reserve Your System Now
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Our Process

To support the growing demand for mRNA based vaccines and therapeutics, NTx has developed a first of its kind, proprietary continuous-flow, in-vitro, transcription system utilizing hollow fiber bioreactors. This biosynthetic process provides a low cost and rapidly deployable vertically integrated manufacturing system. This system allows for manufacturing scale from milligram R&D quantities to large scale commercial needs all in a small, economical footprint, with the supply of critical enzymatic components secured by NTx.
 
All enzymes used in the NTxscribe® platform are engineered with an affinity tag, enabling continuous capture and wash through of all processing steps. In our standard workflow, 5’-capping of the mRNA is accomplished in continuous flow (directly coupled mRNA synthesis).
 

This unique approach minimizes handling of the material in between production steps, prevents damaging the molecules and ensures integrity, incorporates a purification process that improves yields, and can be scaled up linearly using the same process from discovery through product commercialization, saving valuable time.

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The Principal Advantages of NTxscribe® Include:

  • Higher RNA integrity out of IVT compared to batch reactions due to short residence time in bioreactor - reducing burden on downstream purification
  • Complete set of NTx enzymes for mRNA production
  • Single-use, closed system configuration to ensure product sterility
  • Decreased shear and handling, minimizing product degradation and contamination
  • Streamlines manufacturing operations by reducing batch unit operations
  • Enables linear scaling from mg to gram scale
  • Benchtop modular design can accommodate different drug substance formats in small scalable footprint
  • Allows for integration of in-line process analytics

Producing Conventional and Self Amplifying mRNA

Messenger RNA (mRNA) is a single-stranded RNA molecule that carries the genetic sequence of a gene to a ribosome, the protein synthesis machinery of a cell. The ribosome simultaneously reads the mRNA and synthesizes the corresponding protein encoded by the sequence. All cells need  to routinely make mRNA and proteins to regulate protein levels within the cells. Infectious diseases are often also recognized through the immune system by detecting foreign mRNA molecules. Exploiting mRNA as a fast response vaccine candidate was pivotal in the COVID-19 pandemic and will continue to be a powerful solution to combat many diseases of the modern age. NTxscribe® excels across a broad range of mRNA transcript sizes, with a distinguishing ability to produce high integrity saRNA.