DNA Template Calculator
Calculate the amount of DNA template required for your NTxscribe® in vitro transcription (IVT) reaction.
Enter your linearized DNA template length and concentration. The calculator will determine the amount of DNA template required per NTxscribe® run.
i All other parameters are set to default values for NTxscribe® reactions.
DNA Template Required
in milligrams per NTxscribe® Run
in milligrams per NTxscribe® Run
—
mg
DNA Template Required
in mL per NTxscribe® Run
in mL per NTxscribe® Run
—
mL
NTxscribe® Reaction Mix (per run)
| Reagent | Amount (mL) | Final Concentration |
|---|---|---|
| Nuclease-free water | — | N/A |
| NTxscribe® Buffer | 1.7 | 1x |
| Cap Analog (optional) | 0.68 | 4 mM |
| DNA Template | — | 0.05 µM |
| NTxscribe® Enzyme Mix 1 | 3.4 | 2x |
| Total Reaction Volume | 17 |